You need to do Four 10 fold dilution to a volume of 1mL to get 10^2 cells/mL final concentration. You have a bacterial culture, which has 10^6 bacterial cells and you want 100 cells/mL how to dilute the culture to get 100cells/mLįinal Concentration Required - 100 cells/mL or 10^2 cells/mL Serially dilute the bacterial culture, Bacterial culture is generally serially diluted for plating (for bacterial isolation), for re-inoculation or for other applications like checking optical density, etc. How to do Serial Dilution of Bacterial Culture?ĭilution of bacterial culture can be performed the same way as explained above. Stock solution is 1 M, after 1000 fold dilution it becomes - 1/1000 = 0.001 M. Label the tube as Dilution 1.įrom Dilution 1 tube take 10mL and mix 90mL of diluent and Label it as Dilution 2.įrom From Dilution 2 tube take 10mL and mix 90mL of diluent and Label it as Dilution 3.Īs we have done 10 fold dilutions, the tube 3 (Dilution 3) will be 1000 fold diluted than the stock. Mix 10mL of 1M stock with 90mL of Diluent. To do a 10 fold dilution of 1M Stock solution to 100mL, Since we need to do 10 fold dilution, dilution factor is 10.ĭiluent Volume required = 100mL - 10mL = 90mL. Lets see how to serially dilute 1 M stock solution to a final volume of 100 mL.ĭilution Factor = Final Volume / Volume of Stock. 10 fold dilution is actually 10^-1 dilution (Inverse of Dilution Factor: 1/10).ĭiluting a 1 M (Molar) Stock solution 1000 fold by serial Dilution.ġ000 fold serial dilution can be done by three 10 fold dilutions. Dilution is the inverse of Dilution Factor. The ratio of the Final volume to the aliqout volume of the stock is known as Dilution Factor (DF). To dilute a stock solution, Mix the stock solution with diluent.

How to do dilution series calculations